Composite

Part:BBa_K2762007:Design

Designed by: LONG CHEE YUN   Group: iGEM18_NCKU_Tainan   (2018-09-29)


PlacI-B0034-prk-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The whole genome sequence of Synechococcus elongatus PCC7942 is found in NCBI. We first codon optimized the prk sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning.

Source

Synechococcus elongatus PCC7942

References